There are many other ingredients in epimedium extract as well, such as flavones, Quercetin, Ikarioside A and B (wanshanense), Anhydroicaritin, Desmethylanhydroicaritin, Sagittatoside B , Diphylloside A and B, etc. Flavonoids are the main active chemical constituents of Epimedium species, the four major flavonoids contributing to their effects being epimedins A, B, and C, and icariin. These biologically active constituents are flavonol glycosides with an isoprenyl group substituted on carbon 8. However, analysis of natural health products currently available on the market containing Epimedium relys mostly on the presence of icariin. CPC established characteristic Rapid Resolution Liquid Chromatography (RRLC) profiles for complex mixtures of flavonoids from Epimedium species. The Zorbax Eclipse XDB column is able to fully separate four major flavonoids, which are epimedins A, B, and C, and icariin, for both quantitative and qualitative analysis in less than 3 minutes.