L-5-Methyltetrahydrofolate Calcium (L-5-MTHF-Ca)

L-5-Methyltetrahydrofolate Calcium (L-5-MTHF-Ca) is the primary bioactive form of folate in the human body. It directly participates in the one-carbon metabolism cycle without requiring conversion by the MTHFR enzyme.

In biological systems, L-5-MTHF plays a critical role:

• DNA synthesis and repair

• Erythropoiesis (hematopoiesis)

• Regulation of homocysteine (Hcy) metabolism

• Neurotransmitter synthesis (serotonin/dopamine)

Thanks to its high bioavailability and suitability for individuals with MTHFR variants, L-5-MTHF has become a core ingredient in the fields of maternal and infant nutrition, cardiovascular health, mood management, and functional foods.

As a direct manufacturer, Shanghai Aidu Biotechnology Co., Ltd. provides high-purity L-5-MTHF-Ca raw material powder specifically for global dietary supplement brands, nutraceutical OEM manufacturers, and formulation scientists. It offers the same active ingredient with more competitive factory-direct pricing.

Product Description

Appearance: White to pale yellow powder

Solubility: Slightly soluble in water

CAS No.: 151533-22-1

Molecular Weight: 497.52

Molecular Formula:  C₂₀H₂₃CaN₇O₆

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Product Specifications

Product Process and Advantages

· High Activity and High Stability
We adopt an advanced process combining chemical synthesis and biocatalysis to ensure the product's high bioavailability from the molecular source.

1. Highly Selective Bioreduction

Through patented catalytic reduction technology, folic acid is precisely converted into the bioactive L-configuration (levorotatory)

structure. This fundamentally eliminates interference from inactive isomers and significantly enhances human

absorption efficiency.

2. Calcium Salt Crystal Stabilization Technology

By introducing calcium ions to form a stable calcium salt crystal structure, we effectively overcome the technical challenges of easy

oxidation and extreme instability in active folate. This greatly extends the product's shelf life and storage

stability.

· Purification and Quality Control, Benchmarking Top International Standards

Strict Standards: We employ a dual purification technology of multi-stage recrystallization plus ion exchange to precisely remove the inactive D-isomer. It meets the requirements of high-end formulations in Europe and America, with extremely low impurities.

Full-Process GMP: We implement a full closed-loop control from raw materials to packaging, supporting FSSC22000 / HALAL / KOSHER certifications.

· Commercial Supply Advantages: Direct Supply Model, Long-term Empowerment

As a source manufacturer with large-scale production capacity, we provide you with more competitive cooperation solutions.

1. Optimized Cost Structure: Leveraging the economies of scale of our own factory, we eliminate middleman markups, offering significant cost advantages.

2. Highly Stable Supply: Our own production lines ensure sufficient capacity, effectively resisting market fluctuation risks and guaranteeing long-term spot supply.

3. Deep OEM Cooperation: We flexibly support customized needs, making us an ideal partner for long-term brand contract manufacturing and formulation development.

Product Functions and Mechanisms

As a core cofactor in one-carbon metabolism, L-5-MTHF plays a critical role in the methylation cycle:

*Genetic polymorphisms of L-5-methyltetrahydrofolate reductase (MTHFR) and implication in methotrexate depletion of systemic folate.

1.Efficient Anemia Correction and DNA Synthesis:

As illustrated, 5-MTHF is the active endpoint of folate metabolism. It bypasses complex enzymatic reactions to directly supply one-carbon units, ensuring DNA synthesis at the source and preventing anemia.

2. Regulation of Homocysteine (Hcy) Levels:

L-5-MTHF acts as a methyl donor, promoting the conversion of Hcy into methionine, thereby lowering the risks of cardiovascular diseases and pregnancy-induced hypertension.

* L-5-MTHF bypasses metabolic barriers, supporting DNA synthesis to prevent NTDs while directly lowering Hcy levels.

3. Preconception and Pregnancy Support:

It prevents fetal neural tube defects (NTDs) and is unaffected by MTHFR gene polymorphisms, offering an absorption rate far superior to that of ordinary folic acid.

4. Promotion of Neurotransmitter Synthesis:

It facilitates the synthesis of serotonin, dopamine, and norepinephrine, helping to improve mood and cognitive health. (This action supports the research and development of formulations for mood balance and cognitive health.)

Key Features

High Bioavailability: As a "ready-to-use" form of folate, it bypasses liver metabolic conversion and enters the bloodstream directly upon intake.

Gene-Friendly: It effectively addresses the MTHFR enzyme activity deficiency that affects approximately 30%-50% of the global population.

High Safety: It prevents the accumulation of unmetabolized folic acid (UMFA) in the serum, thereby avoiding the risk of masking vitamin B12 deficiency.

Superior Stability: The crystalline form ensures excellent chemical stability at room temperature, making it suitable for processing into tablets, capsules, and solid beverages.

Applications

Maternal and Infant Nutrition: Pre-pregnancy folic acid tablets, maternal multivitamins, and women's health (PCOS/hormone balance) products.

Cardiovascular Health: Formulation products designed to lower homocysteine levels.

Emotional Support: Supplements aiding in anti-depression and brain health for the elderly.

Functional Foods: Fortified flour, milk powder, and energy drinks.

Typical Formulation Combinations

As the most biologically active form of folate, L-5-Methyltetrahydrofolate Calcium delivers significant synergistic effects when combined with other nutrients. Below are typical formulation recommendations widely adopted in the market:

· Prenatal Support: L-5-MTHF-Ca + Iron + Iodine + Vitamin B12

Directly prevents fetal neural tube defects; ensures efficient blood formation and alleviates anemia during pregnancy.

· Cardiovascular Health (Hcy Management): L-5-MTHF-Ca + Vitamin B6 (P-5-P) + Vitamin B12 (Methylcobalamin) + Betaine (TMG)

Synergistically lowers homocysteine (Hcy) levels to prevent stroke and hypertension.

· Cognitive & Mental Health: L-5-MTHF-Ca + SAMe + Magnesium + Omega-3 (DHA/EPA)

Boosts the synthesis of serotonin and dopamine, aids in anti-depression, and protects cognitive function in the elderly.

· Male Fertility: L-5-MTHF-Ca + Zinc + Selenium + L-Carnitine

Safeguards sperm DNA integrity and enhances sperm quality and vitality.

· Premium Anti-aging: L-5-MTHF-Ca + NMN + Coenzyme Q10 + Vitamin D3

Provides deep support for DNA repair and methylation balance, boosting cellular energy from the source.

Packaging & Storage

Packaging Specifications: 1kg/aluminum foil bag, 5kg/aluminum foil bag, 25kg/fiber drum; packaging specifications can be customized according to customer requirements.

Storage Conditions:   Keep sealed, protected from light, in a low-temperature, dry (recommended 2–8°C), and well-ventilated place.

Shelf Life: 24 months

Why Choose Us as Your L-5-MTHF Supplier

We are a global leader in L-5-MTHF-Ca (Active Folate) manufacturing, dedicated to women’s health, prenatal nutrition, and metabolic regulation. Leveraging advanced biotransformation and purification, we provide premium raw materials for the global nutraceutical and pharmaceutical industries.

 

Core Product Matrix：

· L-5-MTHF-Ca (Active Folate)

High Bioavailability: Overcomes MTHFR genetic limitations, enabling direct absorption without conversion.

· 40:1 Inositol Compound Formula (MI/DCI)

Golden Synergy Ratio: Adheres to the 40:1 physiological ratio in human plasma, scientifically regulating insulin sensitivity and facilitating ovulation restoration in PCOS.

· D-Chiro Inositol (DCI) 98%

Focused on 98% high purity, effectively regulates metabolic balance and specifically lowers androgen levels caused by PCOS.

· Myo-Inositol

Preserves egg quality and endocrine health, serving as a classic foundational ingredient for preconception and women's health products.

Core Advantages:

• Integrated R&D, production, and supply chain system
• Stable large-scale production capacity with consistent quality
• Strict multi-level quality control with authoritative international certifications
• Customized product solutions for diverse application scenarios
• Extensive export experience and reliable global supply

AIDU Biotech, a direct source factory, supplies high-purity active ingredients, with rapid delivery from US warehouses, empowering global women's health brands.

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References:

[1] Bailey SW, Ayling JE. The extremely slow and variable activity of dihydrofolate reductase in human liver and its implications. Proceedings of the National Academy of Sciences, 2009.

[2] Obeid R, Herrmann W. Mechanisms of homocysteine neurotoxicity in neurodegenerative diseases. Clinical Chemistry and Laboratory Medicine, 2006.

[3] Homocysteine Studies Collaboration. Homocysteine and risk of ischemic heart disease and stroke. JAMA, 2002.

[4] Bailey LB, Gregory JF. Folate metabolism and requirements. Journal of Nutrition, 1999.

[5] Crider KS, Bailey LB, Berry RJ. Folic acid and risk of neural tube defects. Birth Defects Research, 2011.

[6] De-Regil LM et al. Effects of folic acid supplementation on pregnancy outcomes. Cochrane Database of Systematic Reviews, 2010.

[7] Bottiglieri T. Folate, vitamin B12, and neuropsychiatric disorders. Nutrition Reviews, 1996.

[8] Stahl SM. L-methylfolate: a vitamin for your monoamines. CNS Spectrums, 2007.

[9] Obeid, R., Holzgreve, W., & Pietrzik, K.F. (2013). Is 5-methyltetrahydrofolate an alternative to folic acid for the prevention of neural tube defects? Journal of Perinatal Medicine.